The place De Bilt counts 65 registrations in the national register. Below is an overview. Also see
fast, highly specific and mild-acting biocatalysts in chemical transformations for quantitative analysis. Since these transformations play the role of four factors (substrate, enzyme, activator and inhibitor), one factor may be determined by calibration, provided that the remaining factors are kept constant. Types of analysisIn embodiment, stoichiometric and kinetic enzymatic analysis is distinguished; The former is in simple form only applicable to substrate.
Characteristic especially for the kinetic process is besides speed its high sensitivity, so that high demands are made on the purity of the enzymes and other required ingredients. The result of the enzymatic conversion is usually determined spectrophotometrically (inter alia with a chromogenic substrate), but often also fluorometrically (inter alia with a fluorogenic substrate), electroanalytic, sometimes volumetric (titration or gas development in wartburg equipment) or radiochemical. Frequently, one uses a coenzyme (NADH and the like) and linked enzyme reactions.
The analysis of the enzymes themselves (enzyme analysis) can also be counted on the enzymatic analysis by virtue of, in principle, the same embodiment. many provisions in clinical chemistry (for example, the determination of alkaline phosphatase in blood serum) belong to this. Applications
Some areas of application for enzymatic analysis are: clinical chemistry, food control, detection of trace metals (as activator or inhibitor) and insecticides (as irreversible inhibitor).
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